How do metal cations affect the stability and structure of phospholipid bilayers? Four batches of spray dried PLs were investigated. It will provide a better insight to the generic manufacturers and will help them to identify the critical quality attributes during the formulation development phase. Force spectroscopy with AFM has emerged as a powerful technique able to provide valuable insights into the nanomechanical properties of supported lipid membranes at the nanometer/nanonewton scale in a wide variety of systems. to the length of the lateral alkyl chain. Find more information on the Altmetric Attention Score and how the score is calculated. All rights reserved. In contrast, liposomes composed of lipids with transition temperatures>28°C bound to venous parts of the vasculature. Their traditional representation as a passive 2D fluid has gradually been abandoned in favor of a more complex picture: an anisotropic time-dependent viscoelastic biphasic material, capable of transmitting or attenuating mechanical forces that regulate biological processes. Correlative and synchronous use of different microscopies relies on the fundamental assumption of non-interference during images acquisitions. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Here we present a combination of atomic force microscopy (AFM) imaging, force measurements, confocal fluorescence imaging and two-focus scanning fluorescence correlation spectroscopy (two-focus SFCS) to obtain structural and dynamical information about this model membrane system. Increased methyl-β-cyclodextrin levels and incubation time could significantly increase Young’s modulus and degree of stiffing on cell membrane and cytoskeleton. The increase in the yield threshold force has been related to the increase in lateral interactions (higher phospholipid-phospholipid interaction, decrease in area per lipid) promoted by ions bound into the membrane. Here, we explore the model system DPPC:Chol by means of temperature-controlled atomic force microscopy (AFM) imaging and AFM-based force … This is especially important in contact-dependent signaling, where ligand-receptor binding occurs at cell-cell or cell-ECM junctions. Preclinical evaluation of these nanoaggregates is discussed with a special focus on the investigation of their mechanism of action at a molecular level, highlighting their pharmacological potential in tumour disease models and value for biomedical applications. AS1411-decorated niosomes as effective nanocarriers for Ru( Overall, the notion that in biomembranes selected lipids could laterally aggregate to form more ordered, detergent-resistant lipid rafts into which glycosphingolipids partition is strongly supported by this study. ... At 39 mol % cholesterol no phase transition is recorded, as the membrane enters a lowpermeability liquid-ordered phase, explaining the prolonged release rate of the collagenase ( Figure 1F). PHASE TRANSITION OF CHOLESTEROL H.L. The combination of AFM topography and nanomechanical mapping with specific probes for molecular recognition of lipids represents a novel approach to identify lipid-enriched domains in supported bilayers and offers a unique perspective to directly observe lipid assemblies in living cells. These force maps, while containing rich mechanical information, require lengthy processing time due to the large number of force curves needed to attain a high spatial resolution. Claudia Riccardi, Carme Fàbrega, Santiago Grijalvo, Giuseppe Vitiello, Gerardino D’Errico, Ramon Eritja, Daniela Montesarchio. The potential therapeutic utility of the NO-releasing liposomes was further assessed in biological/proteinaceous fluids. The selectivity of interaction of cholesterol with glycerophospholipids of varying headgroup charge was studied by comparing the cholesterol-induced changes in the Amax values of derivatives of phosphatidylcholine, phosphatidic acid, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylserine spin-labeled at the fifth position of the sn-2 chain. We aim at identifying molecular parameters affecting LSCs viability as key elements to design future anti-cancer drugs that are, less toxic and more effective against CSCs of recurrent leukemia. Cer-rich gel-phase domains were not observed, in contrast to results reported by either direct Cer incorporation or enzymatic Cer generation. Next, the in vitro tumor penetration and the in vivo anti-tumor effects were analyzed. Biophys. The collagozome pretreatment allowed increased drug penetration into the pancreas and improved PDAC treatment. Antibiotics That Affect the Membrane and Other Structural Targets. In this paper, the available binary phase diagrams of lipid-cholesterol mixtures are all collected together. The release of N-palmitoyl and N-butanoyl-d-erythro-sphingosine (C16- and C4-Cer) by the photolysis of caged Cers using long-wavelength UV light was studied using a combination of atomic force, How do metal cations affect the stability and structure of phospholipid bilayers? Impact of galactosylceramides on the nanomechanical properties of lipid bilayer models: an AFM-force spectroscopy study. doi: 10.7554/eLife.52535. Large-sized nano- discs can be produced by first decorating the inside of DNA barrels with small lipid-bilayer nanodiscs, which open up when adding extra lipid to form large nanodiscs of diameters ~45 or ~70 nm as prescribed by the enclos- ing barrel dimension. The vesicle size (d50-value between 3 and 7 µm) was dependent on the size of microparticulated mannitol and not on the size of PLs. Phosphatidylcholine/water 4 µmol/ml is achieved at a temperature of 10°C below the phase-transition temperature of phospholipids ensuing suitability for thermolabile entities and high entrapment efficiency. How do cell walls remodel during cell growth or incubation with drugs? It was determined that cholesterol binds to phosphodiester and alkyl glycerol ester moieties, likely via hydrogen bonding of the alcohol to either of the ester oxygens. 624, The main objective of the project is to directly measure the electron transfer process between partner redox proteins of the human respiratory chain at the single molecule level and under control, The effect of temperature on the lateral structure of lipid bilayers composed of porcine brain ceramide and 1-palmitoyl 2-oleoyl-phosphatidylcholine (POPC), with and without addition of cholesterol, were studied using differential scanning calorimetry, Fourier transformed infrared spectroscopy, atomic force microscopy, and confocal/two-photon excitation fluorescence microscopy (which included, 6-Bromo-7-hydroxycoumarin (Bhc)-caged ceramide (Cer) analogs were incorporated into supported lipid bilayers containing a mixture of coexisting liquid-ordered (L(o)) and liquid-disordered (L(d)) phases. Several cellular processes, including adhesion, signaling and transcription, endocytosis, and membrane resealing, among others, involve conformational changes such as bending, vesiculation, and tubulation. Atomic force microscopy (AFM)-based experimental approaches have been to date very valuable to deepen into these aspects. Nanomechanics of Lipid Bilayers: Heads or Tails? Les limites des nanovecteurs commerciaux ou actuellement en développement ont motivé l’élaboration de nouvelles nanoparticules mésoporeuses de silice (MSNP), hybrides et multiphasées, pour le contrôle de la délivrance d’actifs à application théranostique.
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